Cryopreservation of Arabidopsis Cell Suspensions by Vitrification

Abstract: Cell suspensions are valuable materials for plant physiology and biochemistry studies. To keep the genetic stability of plant cell suspensions， the cryopreservation technique is required. Vitrification is a method of cryopreservation without the usage of programmable freezers. In the present paper， cell suspensions from Arabidopsis， a model plant， were established and cryopreserved by vitrification. After suitable preculture and dehydration treatment， cells were quenched into liquid nitrogen directly. Thawed cells were able to recover， and recovered cells were able to regenerate plants. This is the first report of cryopreservation of Arabidopsis cell suspension cells by vitrification.

CSTR: 32200.14.cjcb.2004.01.0017