Dynamic Observation of the Nucleus Translocation of STAT3 in Senescent Cells Stimulated with Angiotension Ⅱ

Abstract: To investigate the dynamic nuclear-translocation change with aging of human signal transducer and activator of transcription 3 (STAT3) in normal human fetal lung diploid fibroblast cell line， WI-38， stimulated with angiotensin Ⅱ (10-6 mol/L)， we constructed the plasmid pEGFP-hSTAT3 by subcloning of the hSTAT3 cDNA full sequence from pMS1-hSTAT3 into pEGFP-C3， following which transfections through the effectene were conducted into WI-38 cells of the 19th passage and 42^nd passage， respectively， and then observed using the laser scanning confocal microscopy (LSCM). Our results revealed: (1) that after exposure to angiotensin Ⅱ， WI-38 cells took on an accumulation of STAT3 in the nucleus from the cytoplasms; (2) that in cells of the 19^th passage， STAT3 nuclear accumulation began 15 and peaked 30-45 minutes after addition of angiotensin II， while in the 42^nd passage cells STAT3 nuclear accumulation began 30 and peaked 50-60 minutes after stimulation. Taken together， our results demonstrated that STAT3 nuclear translocation were delayed by cellular senescence in WI-38 cells， which may interfere with the cell proliferation and be one of the mechanisms of aging.

CSTR: 32200.14.cjcb.2004.01.0016