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Membrane Damage in Rat Striatum Neurons by Heat Treatment
ZHAO Yong Qi1, LIU Shu Hong1, ZHAO YU Lan2, Wu Yan1, GE Xue Ming1, LAO Jie2, FAN Ming1*
1Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing 100850, China; 2Medical Experiment & Analysis Center of PLA General Hospital, Beijing 100853, China
Abstract: This work was aimed to inspect the effect of heat treatment on the membrane metabolism and fluidity of rat striatum neurons， and to probe the main reason of heat injury on neuron. Neurons from rat striatum were primarily cultured. After treatment at 43℃ for 1 h， the level of intracellular fatty acids and level of fatty acid inside cell membrane， especially the level of arachidonic acid， were mensurated by Gas/Mass chromatography. At the same time， the membrane fluidity was analyzed with fluorescent probe DPH， and the activity of PLA2 was evaluated using [3H] arachidonic acid labeled E. coli membrane substrate. The level of moderate MW fatty acids (C16 and C18) in neurons kept unchanged after 1 h heat treat， while arachidonic acid increased significantly. Membrane fluidity of heat-treated neurons decreased comparing to the control， while activity of intracellular PLA2 was down regulated. We concluded that heat treatment resulted in the decrease of membrane fluidity and disorder of membrane metabolism of neurons， which would be the main cause of injury of heat on these cells.
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