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Real-time Quantitative PCR and Its Applications
WANG Liang Yan1, HONG Qi Hua2*, ZHANG Yao Zhou1
1College of life Sciences, Zhejiang University, Hangzhou 310029, China; 2College of Animal Sciences, Zhejiang University, Hangzhou 310029, China
Abstract: Developed in the mid 1990s for the analysis and quantification of nucleic acid， real-time quantitative PCR is a molecular biological technique gaining rapidly in popularity. The advancement provided by the real-time version of PCR is due to its unique ability to monitor the complete DNA amplification progress and other features such as rapidness， sensitivity and accuracy. Real-time Quantitative PCR has reformed the conventional PCR as well as enlarged applications. This article reviews the principle， instruments， detection systems for quantification and some applications of this new technique.
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