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Establishment and Characterization of Embryo Cell Line of Bombyx mori-SWU1
Min-Hui Pan, Shi-Quan Xiao, Xi-Jun Hong, Cheng Lu*
Key Sericultural Laboratory of Agriculture Ministry, College of Sericulture and Biotechnology, Southwest University, Chongqing 400716, China
Abstract: Embryo cell line of Bombyx mori-SWU1 (BmE-SWU1) was established after one year primary culture of differentiation and filtration. The cell line was mainly constructed with two types: shuttle and spheroid. And a few prominency and huge cells were found in cultivation. The cell population doubling time was 57.6 h at 28 ℃. The chromosome of BmE-SWU1 cells showed typical characteristics of lepidoteran insect chromosome of metaphase without apparent centromere， and was short pole-like and pellet-like. The longest chromosome was about 4 mm. The cells were heteroploidy. The infection of BmNPV on the different insect lines showed that BmE-SWU1 was more sensitive to BmNPV. TCID50 was 2.92415×10-7.
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