Effects of Co-cultured with Endostatin Gene Transfected Keratinocytes on Proliferative Characteristics of the Human Dermal Microvascular Endothelial Cells

Abstract: Constructed a recombinant endostatin adenovirus expression vectors and transformed these vectors into keratinocytes， co-cultured the kerotinocytes with human dermal microvascular endothelial cells， then determined the content of endostatin in the supernate， the phase percent of the proliferative cycle， apoptosis and the cell inhibition ratio of the endothelial cells. The endostatin expression by keratinocytes reached 226 ng/ml after 3 days of co-culture. The apoptosis percentage and the cell inhibition ratio of the endothelial cells co-cultured with gene-tranfected keratinocytes were significantly higher than those in control group. In addition， the proportion of G0/G1 period was higher than that in control group， while the the proportion of S and G2/M period were on the other way round. Therefore， co-cultured gene-transfected keratinocytes could promote apoptosis and inhibite the proliferation of the endothelial cells through excretion of endostatin.

CSTR: 32200.14.cjcb.2005.06.0024