Optimal Culture Method of the Porcine Preadipocyte

Abstract: Compared the effect of different culture methods， collagenase， mesh aperture and centrifugal force on the swine preadipocyte in primary culture. The results were as follows: the tissue mass and the digested method were all able to outgrow pig preadipocyte， but the mature adipocyte number was less from the tissue mass than the digested method， and the cells were highly homogeneous， proliferative， and differentiating from the digested method， which were mature adipocytes by oil red o staining identification. During the culture by digested method， the Ⅳ collagenase was used for the first time， the 200 item mesh， 800 g centrifugal force， 5 min could get lots of， homogeneous porcine preadipocyte. In conclusion， we established successfully the optimal system of primary porcine preadipocyte culture， and redisplay the whole process of the proliferative and hypertrophy of porcine preadipocyte.

CSTR: 32200.14.cjcb.2005.06.0022