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Cloning， Expression and Purification of a Novel Human GCN5-related Acetyltransferase， MAK3
Hui-Qiang Wang1,2, Ping Li2, Qi-Wei Zhai1*
1Institute for Nutritional Science, Shanghai Institutes for Biological Science, Chinese Academy of Sciences, Shanghai 200031, China; 2Rice Research Institute, Sichuan Agricultural University, Chengdu 611130, China
Abstract: A novel human GCN5-related acetyltransferase， MAK3 was found by bioinformatical analysis of the GNAT family. Human MAK3 contains a conserved acetyltransferase domain and the protein sequence of MAK3 is highly conserved in different species. We detected mRNA level of MAK3 in different tissues by semi-quantity and full quantity RT-PCR and cloned MAK3 into both mammalian and bacterial expression vectors， and the expression of MAK3 was confirmed by Western blot. MAK3 expressed in bacteria was further purified with affinity chromatography. Moreover， the enzyme activity of purified MAK3 protein was also detected.
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