Application of the Single Molecule Detection Techniques in Life Science

Abstract: The single molecule detection technique is a technology to study conformational change， dynamics， interaction and manipulation of single molecule by fluoresce labeling. In ensemble measurements involving a large number of molecules， the dynamic properties of biomolecules and the unique operations of molecular machines are averaged and cannot be observed. Molecule detection techniques have allowed us to record the behavior of individual molecules in real time. The dynamic properties， which were hidden in averaged ensemble measurements， have been unveiled. In recent years， the molecule detection techniques have developed rapidly and opened up a new era of life science. The present review summarized recent progresses in application of molecule detection techniques in molecular motors， DNA transcription， enzyme reactions， protein dynamics， and cell signaling.

CSTR: 32200.14.cjcb.2005.06.0012