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Evaluation of Some Factors Effecting in Vitro Primary Culture of Larval Fat Body Cells of Anoplophora glabripennis
Huan Zhang, Xuan Li, Yong-An Zhang1, Qi-Lian Qin*, Lin Miao, Yu-Zhu Wang1, Liang-Jian Qu1, Fa-Yuan Wen1, Ai-Jun Zang, Qing Yang
State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China; 1Key Laboratory of Forest Protection of State Forestry Administration, Research Institute of Fo
Abstract: Some factors effecting in vitro primary culture of larval fat body cells of the Asian longhorned beetle (Anopolphora glabripennis) were evaluated in the paper. The results showed that the insect stages， size and integrality of the fat body tissue， character of culture surface and whether the cultured tissues attaching to the culture surface were important for the success of the primary culture. Under the optimized conditions， which were in combination with higher larval stages (4th or 8th instar)， more intact cultured tissue and close attachment of the cultured tissue and the culture surface， the fat body cells were inclined to separate from the cultured tissues and proliferate.
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