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The Anti-mutagenicity of Reduced Coenzyme NADH by Ames Assay and Comet Assay
Ju-Yi Wen, Hai Sun, Ji-Ren Zhang*
Department of Oncology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China
Abstract: The anti-mutagenicity of reduced coenzyme NADH was studied by Ames assay and single cell gel electrophoresis assay (SCGE， comet assay). The results showed that the the increasing of the frequency of revertant colonies caused by treating TA98， TA100 with positive mutagens could be inhibited by the middle and high dose of reduced coenzyme NADH， with or without S9 . The comet assay showed that the length of DNA migration (the length of the tail of comet cells) and the percentage of migrated DNA were decreased by the middle and high dose of NADH. It indicates that the reduced coenzyme NADH might be able to inhibit mutagenesis caused by mutagens.
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