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A Comparison of Components and Their Activities of Isozymes between Resting Cyst and Vegetative Cell of the Ciliate Tachysoma pellionella (Protozoa, Ciliophora)
Ji-Wu Chen1, Yi-Song Li1, Hong Zeng1,2, Fu-Kang Gu1
1School of Life Science, East China Normal University, Shanghai 200062, China;
Abstract: Polyacrilamide gel electrophoresis enzyme-chemistry technique analysis revealed that the components of seven species of isozymes including lactic dehydrogenase, a-phosphoglycerol dehydrogenase, alcohol dehydrogenase, cytochrome oxidase, glucose-6-phosphate dehydrogenase, peroxidase and catalase in the resting cyst of Ciliated protozoa Tachysoma pellionella is obvious different from those in the vegetative cell and that these isozymes in the resting cyst has lower activities and less components than those in the vegetative cell. There is trend of simplification of the electrophoretogram patterns for these isozymes in the resting cyst. The components of the other three species of isozymes in the resting cyst, ATPase, malic dehydrogenase and glutamic acid dehydrogenase, are the same as ones in the vegetative cell , but their activities are different and the former lower than the latter.