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Suppression of Bcl-2 Gene Expression with RNAi in SiHaB2 Cell
Xue-Hong Lin1, Geng-Feng Fu1,2, Dan Guo1,2, Xin-Juan Liu2, Jian-Jun Wang1,2, Gen-Xing Xu1,2*
1School of Life Sciences, Nanjing University, Nanjing 210093, China; 2Jiangsu Research Center for Gene Pharmaceutical Engineering and Technology, Nanjing 210049, China
Abstract: Bcl-2 gene， a latent inhibitor of the apoptosis， modulates the death of cells. To suppress the expression of Bcl-2 gene in malignant cells can promote the apoptosis of tumor cells. In this study， we have synthesized a small double-strand interference RNA (siRNA-Bcl-2) containing 21 nucleotides and constructed a plasmid (pSilencer2.1-U6-Bcl-2) containing 19 nucleotides with RNAi. Whereafter， we have transferred both of them separately into the SiHaB2 cells， which highly express Bcl-2 proteins. By the means of Western blot analysis， immunofluorescent analysis and DNA ladder analysis， we have observed that the expression of Bcl-2 proteins in the SiHaB2 cells transferred with siRNA-Bcl-2 or psilencer2.1-U6-Bcl-2 was notably inhibited.
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