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Different Methods of Parthenogenetic Activation on Porcine Oocytes
Jun-He Hu1,2, Xiao-Ming Xu1, Chun-Rong Yang1, Zhong-Ying Dou1*
1Shaanxi Province Branch Center of National Stem Cell Engineering & Technology Center, Northwest Sci-tech University of Agriculture and Forestry, Yangling 712100, China; 2Department of Life Science, Yulin College, Yulin 719000, China
Abstract: Abstract: The effects of ionomycin， eclectrical field strength， numbers of electrical pulse and electrical stimulation in combination with chemical stimulation on parthenogenetic activation of porcine oocytes were studied. The results are as follows: (1) The activation rate with 10 μmol/L ionomycin dealing 5 min (62.97%， 17/27) was not significantly different from that of dealing 10 min (62.50%， 15/24)， 15 min (65.21%， 15/23). (2) The activation rate with three times electrical stimulation of 120 V/mm for 60 μs (66.67%，30/45) was significantly higher than that of electrical stimulation of 60 V/mm (40.98%，17/42)， 80 V/mm (44.11%，15/34)，100 V/mm (46.19%， 18/39)， but was not significantly different from that of 140 V/mm (63.89%，23/36)， 160 V/mm (64.10%， 25/39). (3) The activation rate with two times electrical stimulation of 120 V/mm for 60 μs (67.40%， 31/46) was not significantly different from that of one time electrical stimulation (62.80%， 27/43)， three times electrical stimulation (68.30%， 28/41). (4) The activation rate with three times electrical stimulation of 120 V/mm for 60 μs in combination with 10 μmol/L ionomycin dealing 5 min (84.84%， 29/33) was significantly higher than that of two times electrical stimulation of 120 V/mm for 60 μs alone (67.64%， 23/34). The results of this experiment showed that two times electrical stimulation of 120 V/mm for 60 μs in combination with 10 mmol/L ionomycin dealing 5 min can efficiently activate porcine oocytes.
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