Role of TRF1， TRF2 in Chromosomal Aberrance of MGC803 Cells Induced by Arsenic

Abstract: The purpose of this experiment is to investigate the role of telomeric repeat binding factor1， 2 (TRF1， TRF2) in the process of chromosomal aberrance of MGC803 cells induced by arsenic， and to explore the possible mechanism of cell proliferation and carcinogenesis induced by arsenic. Human gastric cancer MGC803 cells were used in the experiments， the aberrated rate and mitotic index of MGC803 cells were detected by chromosomal analysis. Expression of TRF1， TRF2 and PCNA were detected by Western blot. Both mitotic index and expression of PCNA in MGC803 cells exposure to 0.625 mmol/L As2O3 for 4 week were higher than that of the controls. Chromosomal analysis indicated that the aberrance rates of MGC803 cells were significantly higher than that of the controls， and the most type of aberrances was chromosomal fusion (double or multi-centromere， ringed chromosome). Western blot analysis showed the expression of TRF1 was up-regulated while TRF2 was down-regulated. The present results suggest that the up-regulation of TRF1 and down-regulation of TRF2 could increased chromosomal aberration rate and genome instability in MGC803 cells by long-term exposure to low concentration arsenic.

CSTR: 32200.14.cjcb.2005.05.0019