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Human Basic Fibroblast Growth Factor may Have No Effect on the Phenotype Converse of Human Tenon抯 Capsula Fibroblast
Bing Xie, Wen Ye1*, Yi-Sheng Zhong, Xi Shen
Department of Ophthalmology, Shanghai Jiaotong University Affiliated Ruijin Hospital, Shanghai 200025, China; 1Department of Ophthalmology, Fudan University Affiliated Huashan Hospital, Shanghai 200040, China
Abstract: The purpose of the present study was to explore whether human basic fibroblast growth factor (bFGF) had the effect on phenotypic transition of human subconjunctival Tenon''s capsular fibroblast (HTCF) after operation or trauma. Cultured HTCF derived from 5 human cataract operation cases were induced by bFGF of different concentration (0， 5， 10， 20 ng/ml) for 48 h. Then a-smooth muscle actin (Α-SMA) was detected by immunocytochemistry and Western blot procedure， and it could be observed whether there was differential expression in comparison with normal HTCF. Cultured HTCF stimulated with different concentration of bFGF for 48 h induced that bFGF could not up-regulate the expression of Α-SMA. bFGF from 0-20 ng/ml for 48 h could not induce HTCF transit to myofibroblast in vivo.
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