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In Vitro Culture of Human Saphenous Vein Endothelial Cells
Bin Li*, Nin Liu1
School of Pharmacy , Center for Advanced Studies of Medicinal and Organic Chemistry of Yunnan University , Kunming 650091, China ; 1Department of Obstetrics & Gynecology, the First Hospital of Yunnan Province, Kunming 650032, China
Abstract: The human saphenous vein endothelial cells were successfully dissociated and cultured by using the human saphenous vein segments leftover from cardiac bypass surgery. Endothelial cells were received， proliferated， frozen， and resuscitated by using digestive enzyme. The content of living cells produced using this method is very high and the passage cells were similar to the primary culture in shape and on the growth rate. It was also shown that endothelial cells line in vitro can be established simply and timely using this method.
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