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Generation of pαMHC-EGFP Murine Embryonic Stem Clones
Yan-Qiu Yu*, Hai-Qin Ren1, Hai-Peng Zhang
Department of Pathophysiology, China Medical University, Shenyang 110001, China; 1Shenyang Reproductive Medical Technology Research Center, Shenyang 110005, China
Abstract: We have established a murine D3 embryonic stem cell clone(αMHC-EGFP) with expresses the enhanced green fluorescent protein (EGFP) under the transcriptional control of the alpha-myosin heavy chain (αMHC) promoter， selected ES clones with 200 μg/ml neomycin， cardiomycytes derived from transgenic pαMHC-EGFP embryonic stem (ES) cells with "hanging drop" method in vitro. We have confirmed pαMHC-EGFP ES clones by expression of green fluorescence protein (GFP) in seventh day or eighth day under fluorescence microscope. Meantime compared with D3 ES cells， the change of ratio of cardiomycytes differentiation is not remarkable (P>0.05). So the pαMHC-EGFP murine embryonic stem clone is of great benefit to identify and purify of cardiomyocytes from undifferentiated ES cells.
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