In Vitro Isolation and Culture of Buffalo (Bubalus bubalis) Skin Fibroblast Cells

Abstract: Methods for isolation and culture of buffalo fibroblast cells were studied. The primary buffalo fibroblasts derived from tissue explant culture grown slowly and confluented together by 12 days of culture， while fibroblasts derived from enzymatic digestion grown faster and confluented together by 8 days of culture. However， the growing speed of passaged fibroblasts derived from the two methods was similar， which could confluent within 4 to 5 days of culture. There were 70%-80% of cells survived after frozen and thawed. Karyotype analysis showed that 66.67%-81.67% of fibroblasts had normal karyotype， which did not show significant difference among passages. These results indicated that fibroblast cells can be passaged and keep relatively stable karyotype in vitro.

CSTR: 32200.14.cjcb.2005.04.0020