Effects of Genistein on the Proliferation and Metastasis of Murine Hepatocarcinoma Cell Line with Different Lymphatic Metastatic Potential

Abstract: In vitro， the inhibitory effects of genistein (Gen) on the growth of murine hepatocarcinoma cell lines with different lymphatic potential， HepA-H and HepA-L cells， were evaluated by MTT assay. Gen-inducing apoptosis was observed by scanning electron microscopy (SEM)，transmission electron microscopy (TEM) and flow cytometry (FCM). In vivo， Gen was administered at the concentration of 200 mg/(kg·d)^-1 by intraperitoneal injection daily from the day before HepA-H and HepA-L were injected subcutaneously to foot pad of NIH mice. The popliteal lymph nodes were taken and the metastatic rates were calculated after Gen treatment for 14 days. The apoptosis was checked by using TUNEL method. The results showed that in vitro， Gen exhibited antiproliferative activity. The proliferation inhibition rate of Gen on HepA-H was higher than that on HepA-L. The apoptotic rates induced by Gen were on dosage dependent by using FCM， and the apoptotic rates of HepA-H was higher than that of HepA-L. In vivo， Gen could inhibit the metastasis of tumor cells and reduce the volume of tumor. The prohibition of Gen on the tumor of HepA-H was better than that on the tumor of HepA-L.AI of tumor come from HepA-H (3.87%) was higher than that from HepA-L(1.69%). In conclusion， Gen has a remarkable antitumor activity and could inhibit the tumor cells from invading into lymphtic vessels， especially. The inhibition of Gen on HepA-H is better than that on HepA-L. The antitumor effect of Gen is relative to the induction of tumor cell apoptosis and the therapy of Gen on tumor has selectivity.
    

CSTR: 32200.14.cjcb.2005.04.0019