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Effects of Tourmaline-treated-water on the Growth and the Activity of Alkaline Phosphatase of Caco-2 Cell
Mei-Sheng Xia*, Cai-Hong Hu, Hong-Mei Zhang, Li Xiong, Zi-Rong Xu
College of Animal Science, Zhejiang University; The Key Laboratory of Molecular Animal Nutrition, Ministry of Education, Hangzhou 310029, China
Abstract: Water molecules form an infinite hydrogen-bonded network with localized and structured clustering. The structural change of water clusters may play an important role in physiological processes of life. In this study， structural change of water clusters of distilled water induced by polar crystal tourmaline was observed and caco-2 cells were used to investigate the effects of tourmaline-treated-Dulbecco's Modified Eagle Medium (DMEM) on the cell growth and the activity of alkaline phosphatase. The results showed that tourmaline reduced 17O-nuclear magnetic resonance (17O NMR) full width at halfmaximum intensity (FWHM) for distilled water and the volume of clusters of water molecules. The growth of cell was promoted and the activity of alkaline phosphatase was improved when tourmaline-treated-DMEM was used to incubate the cell. The results indicated the structural change of water clusters induced by tourmaline accounted much for promoted growth and differentiation of the cell observed in this study.
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