Teratogenic Effect of Copper Acetic Acid on Vicia faba Root Tip Cells

Abstract: With different concentrations of copper acetic acid as mutagen， we used the micronucleus assay and chromosome aberration assay to determine mitotic index， micronucleus rate and chromosome aberration rate of Vicia faba root tip cells. The result showed that copper acetic acid could increase the micronucleus rate of Vicia faba root tip cells. Within certain range of concentration of copper acetic acid and treating for 6 h and 12 h， the rate of micronucleus was found to increase systematically with the added concentration of copper acetic acid. Within certain range of concentration of copper acetic acid and treating for 24 h， the rate of micronucleus was found to increase systematically with the added concentration of copper acetic acid， and then to decrease systematically with the concentration. At different time of treatment， different concentrations of copper acetic acid could apparently increase mitotic index. Copper acetic acid also caused high rate and various types of chromosome aberration. Thus， copper acetic acid had systematical teratogenic effects on Vicia faba root tip cells.

CSTR: 32200.14.cjcb.2005.03.0024