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The Effects of Deoxynivalenol on LMP-2 Expression of Human Peripheral Blood Mononuclear Cells in Vitro
Yue-Hong Li, Xiang-Hong Zhang*, Ling-Xiao Xing, Lian-Fu Zuo1, Xia Yan, Jun-Ling Wang, Feng-Rong Wang
Laboratory of Experimental Pathology, Institute of Basic Medicine, Hebei Medical University, Shijiazhuang 050017, China; 1Laboratory of Cytology, Institute of Hebei Province Oncology, Shijiazhuang 050011, China
Abstract: To explore the effects of deoxynivalenol (DON) on low molecular weight polypeptide-2 (LMP-2) expression of human peripheral blood mononuclear cells. Effects of deoxynivalenol at different concentration on LMP-2 expression of human peripheral blood mononuclear cells in vitro were studied with flow cytometry (FCM) analysis and RT-PCR. FCM analysis indicated that LMP-2 expression of human peripheral blood mononuclear cells were reduced at protein level in DON treatment groups (50 ng/ml， 100 ng/ml， 1000 ng/ml and 2000 ng/ml)， the mean fluorescence intensity of LMP-2 expression in DON groups were 6.99±0.72、6.21±0.55、5.34±0.56 and 5.03±0.43， respectively. As the DON concentration increase from 50 ng/mL to 2000 ng/mL， the expression of LMP-2 was decreased correspondingly and a significant dose-depended response negative correlation could be found between expressions of LMP-2 and DON concentration. RT-PCR also confirmed that DON at higher concentration (1000 ng/ml and 2000 ng/ml) could distinct inhibit LMP-2 mRNA expressions. The results suggest that DON could reduce LMP-2 expressions of human peripheral blood mononuclear cells at protein and mRNA level in a concentration-depended manner in vitro.
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