Home > Browse Issues > Vol.27 No.3
The Effect of Polypeptide Extract from Scopion Venom on Angiogenesis in Vitro
Wei-Dong Zhang*, Ya-Zhou Cui 1, Li-Cun Wu, Qing Jia, Shou-Qin Song, Zhao-Xia Wang, Qiang Dong
Department of Pathology, Shandong Academy of Medical Sciences, Jinan 250062, China; 1Shandong Medical Biotechnological Center, Jinan 250062, China
Abstract: The study was designed to evaluate the anti-angiogenic activity in vitro of PESV， a peptide extract from scorpion venom. Human umbilical vascular endothelial cell (HUVEC) was used to test the anti-angiogenic effect of PESV in vitro. HUVEC proliferation was detected by the BrdU cell incorporation ELISA. Apoptosis level and expression of Bcl-2 and Bax protein of HUVEC was determined by flow cytometry. Chicken embryo chorioallantoic membrane (CAM) assay was used to determine the effect of PESV on neovascularization. PESV exhibited potent anti-proliferative and apoptosis-induced activity against HUVEC in vitro. In addition， PESV demonstrated preferential inhibition of endothelial cells. PESV also demonstrated suppression of neovascularization in the CAM assay. Our data suggest that PESV is of potent anti-angiogenic activity in vivo. PESV could be regarded as a candidate for tumor angiogenesis inhibitor (TAI) and a potent chemotherapeutic agent of malignant tumors， and its component should be further analyzed.
CN
EN