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Establishment of Insulin-resistant HepG2 Cell Model Induced by High Concentration of Insulin
Qiu Chen*, Yong-Peng Xia, Zong-Yin Qiu
Department of Pharmacology, Chongqing Medical University, Chongqing 400016, China
Abstract: To establish insulin resistant HepG2 cell model induced by high concentration of insulin in vitro culture. HepG2 cells were incubated with 5×10-7 mol/L insulin for 24 hours， washed with DMEM and finally with pre-cold PBS. The residual 125I-insulin binding and the insulin-stimulated 3H-D-glucose incorporation were determined. It was demonstrated that the residual 125I-insulin binding and incorporation rate of 3H-D-glucose in insulin-resistant HepG2 cells were decreased significantly than that in control cells. Insulin-resistant HepG2 cells were free from stimulation for 60 hours by insulin， but the incorporation rate of 3H-D-glucose were remaining lower than that in control cells. HepG2 cells exposed to 5×10-7 mol/L insulin for 24 hours were able to induce a state of insulin resistance and reproduce a insulin-resistant cell model. The state of insulin resistance could be maintained for 60 hours. The method is simple， practicable and reliable.
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