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Localization of Insulin-like Growth Factor I Receptor in Caveolae of Neuronal Cells
Xiu-Li Lu, Xiang-Yu Cao, Jian-Li Liu, Fang-Fang Hou, Zhen-Qing Liu, Bing Gao1*
The School of Life Science, Liaoning University, Shenyang 110036, China; 1The Institute of Basic Medical Sciences, Shenyang Medical College, Shenyang 110034, China
Abstract: Insulin-like growth factor-I (IGF-I) is the important factor which regulates the growth of neuron. Caveolae， cholesterol-rich microdomains of plasma membrane， act as platforms for some neurotrophic factors. In this study， we examined the relationship of IGF-I to caveolae in neuronal cells. The neuronprotective action of IGF-I was inhibited by the methyl-β-cyclodextrin (CD)-induced cholesterol depletion in pheochromocytoma PC12 cells. Immunocytochemical analysis demonstrated that IGF-I receptor was localized in caveolae; treatment of CD destroyed the localization of IGF-I receptor in caveolae. The primary culture of neuron further shown that the anti-apoptotic role of IGF-I was also involved in cholesterol content. Cerebellum granules neuron culture demonstrated that IGF-I receptor was localized in both soma and neutrite parts of neuron. Taken together， our results demonstrated that IGF-I receptor was localized in caveolae of neuron which was required for the neuroprotective action of IGF-I.
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