Effects of LncRNA OIP5-AS1 on the Proliferation,Invasion and Epithelial-Mesenchymal Transitionof Nasopharyngeal Carcinoma Cells by Targeting miR-381-3p/FSCN1 Axis
ZHANG Yi, WANG Li, HUANG Yongyang*
This article aimed to investigate the effects of LncRNA OIP5-AS1 on the proliferation, invasion and EMT (epithelial-mesenchymal transition) of NPC (nasopharyngeal carcinoma) cells by targeting themiR-381-3p/FSCN1 axis. The expression levels of LncRNA OIP5-AS1 and miR-381-3p, as well as the protein expression level of FSCN1, were detected in cancerous tissues and paracancerous tissues of NPC patients. The HNE1 cells were assigned into the sh-NC group, the sh-OIP5-AS1 group, the miR-NC group, the miR-381-3p mimicgroup, the sh-OIP5-AS1+inhibitor NC group, and the sh-OIP5-AS1+miR-381-3p inhibitor group. The RT-qPCR experiment was used to measure the expression levels of LncRNA OIP5-AS1, miR-381-3p and FSCN1 mRNA. MTT assay was implemented to detect the cell proliferation ability. Plate colony formation assay was implementedto measure the number of cell colonies. Transwell assay was used to detect cell migration and invasion abilities.The dual luciferase reporter gene assay and RIP experiment were used to detect the direct binding relationshipbetween miR-381-3p and FSCN1, as well as between miR-381-3p and LncRNA OIP5-AS1. Western blot experiment was used to measure the expression levels of E-cadherin, CK18, Slug and FSCN1 proteins. The nude mousetumor transplantation model was constructed and assigned into the Control group, the sh-NC group, and the shSNHG14 group. The mass and volume of the transplanted tumors were measured, and the expression of FSCN1,Ki-67, E-cadherin and MMP9 proteins were detected by immunohistochemistry. Compared with the negative control groups, the cell D490 value, the number of clone formations, the number of migrated and invaded cells, and the expression levels of FSCN1 and Slug proteins in the sh-OIP5-AS1 group and miR-381-3p mimic group were lower, while the expression levels of E-cadherin and CK18 proteins were higher (P<0.05). Compared with the sh-OIP5-AS1+inhibitor NC group, the cell D490 value, the number of clone formations, the number of migrated and invadedcells, and the expression levels of FSCN1 and Slug proteins in the sh-OIP5-AS1+miR-381-3p inhibitor group werehigher, while the expression levels of E-cadherin and CK18 proteins were lower (P<0.05). Animal experimentsshowed that knocking down LncRNA OIP5-AS1 inhibited the growth of transplanted tumors, decreased the expression levels of LncRNA OIP5AS1 and FSCN1 mRNA, as well as the protein expression of FSCN1, Ki67, andMMP9, while increased the expression level of miR3813p and the protein expression of Ecadherin (P<0.05).LncRNA OIP5-AS1 promotes the proliferation, invasion and EMT process of NPC cells by targeting and regulatingthe miR-381-3p/FSCN1 axis.
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