(S)-4-Methoxydalbergione Suppresses Hepatocellular CarcinomaProgression by Upregulating FGB Expression
WANG Yan, FENG Zhenbo*
This study investigates the biological function of S-4MOD [(S)-4-methoxydalbergione] in suppressingthe development of HCC (hepatocellular carcinoma) by upregulating FGB (fibrinogen beta chain) expression, aiming to reveal the molecular mechanisms underlying HCC development and progression. The functionally revlevant target FGB of S-4MOD was screened using public databases and transcriptome sequencing results. The effect of S-4MOD on FGB mRNA expression levels in HCC cells was detected by qRT‑PCR. In this study, a transient siRNA transfection approach was usedto establish an FGB-knockdown (siFGB) cell model. qRT-PCR was then performed to detect FGB mRNA expressionlevels and to verify the knockdown efficiency. Subsequently, cell proliferation was assessed using CCK‑8 and colony formation assays; cell migration was evaluated by wound healing and Transwell assays; and cell apoptosis was detected byHoechst 33258 fluorescent staining. These experiments collectively allowed a systematic evaluation of the impact of FGBknockdown on the anti‑malignant phenotype effects of S-4MOD in HCC cells. Results from qRT-PCR showed that S-4MOD significantly upregulated the mRNA expression level of FGB in HCC cells (P<0.001). CCK8, colony formation, woundhealing and Transwell assays confirmed that knockdown of FGB expression significantly attenuated the inhibitory effects of S-4MOD on HCC cell proliferation and migration (P<0.05). Hoechst 33258 staining revealed that knockdown of FGB expression significantly reduced the proapoptotic effect of S-4MOD on HCC cells (P<0.05). The expression level of FGBis closely correlated with the anti-HCC activity of S-4MOD. Knockdown of FGB expression significantly attenuates thetherapeutic efficacy of S-4MOD against HCC.
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