Impacts of LncRNA MIR17HG on Proliferation and Invasion of MyelomaCells by Targeting miR-153-3p
AN Hongyu, ZHANG Xin*
This article aims to explore the effects of LncRNA MIR17HG targeting miR-153-3p on the proliferation and invasion of MM (myeloma) cells. qRT-PCR method was used to detect MIR17HG and miR-153-3p in MM tissue, normal bone marrow tissue, nPCs (human normal bone marrow plasma cells), and MM cells (U266, RPMI-8226, NCI-H929, LP-1). NCI-H929 cells were assigned into control group, si-NC group, si-MIR17HG group, si-MIR17HG+inhibitor NC group, si-MIR17HG+miR-153-3p inhibitor group, si-MIR17HG+miR-NC group, and si-MIR17HG+miR-153-3p mimics group. qRT-PCR was performed to determine the expression levels of MIR17HG and miR-153-3p in cells. The CCK-8 assay was conducted to assess cell proliferation. A wound healing assay was used to evaluate cell migration. A Transwell assay was carried out to examine cell invasion. Flow cytometry was employed to detect cell apoptosis. Western blot was used to detect the protein expression levels of E-cadherin, cleaved caspase-3, N-cadherin, vimentin, PCNA, and MMP-2 in cells. The targeting relationship between miR-153-3p and MIR17HG was validated. The MIR17HG increased and miR-153-3p decreased in MM tissues and MM cell lines. After knockdown of MIR17HG expression, the expression level of MIR17HG in the NCI-H929 cells, the values of D450 (24 h, 48 h) , the scratch healing rate, the protein expression levels of N-cadherin, vimentin, PCNA and MMP-2 , and the number of cell invasions reduced, while the expression level of miR-153-3p, apoptosis rate, the protein expression levels of E-cadherin, and cleaved caspase-3 increased (P<0.05). Silencing miR-153-3p could reduce the inhibitory effect of knocking down MIR17HG on the malignant biological behaviors of NCI-H929 cells (P<0.05). Moreover, upregulation of miR-153-3p could enhance the inhibitory effect of knocking down MIR17HG on the malignant biological behaviors of NCI-H929 cells (P<0.05). MIR17HG can regulate the expression of miR-153-3p and affect the malignant biological behavior of MM cells.
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