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Primary Culture of Calf Preadipocyte
Cheng Xia, Zhe Wang1*, Shu-Ling Niu1, Cai Zhang2, Hong-You Zhong
College of Animal Science, HeiLongjiang Aug.1st Agriculture University, Daqing 163319, China; 1 Department of Military Veterinary, Liberation Army Required University, Changchun 130062, China; 2College of Animal Science and Veterinar
Abstract: To establish a primary calf preadipocyte culture method for better understanding the properties of hyperplasia of calf adipose tissue， fibroblast like cells from calf small intestine omentum were cultured and fibroblasts from cattle dermise were also cultured to serve as control. Results shown: The cells from intestine omentum were highly homogeneous， proliferative and have high differentiation rate. Their dynamic morphological changes， growth curve， extracting stained intracytoplasmic lipid with oil red O， all verified their preadipocyte identity. Under controlled conditions， the preadipocytes replayed their hyperplasia process in vitro. Conclusion: In calf omentum tissue， there exist preadipocytes that can differentiate into mature adipocytes. Since adipocyte is one kind of classic target cells to insulin， this study aid the basis for further probing into obesity and insulin resistant diseases such as ketosis and fatty liver in dairy cows.
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