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Transfer of Arabidopsis CBF1 Gene Leads to Increased Proline Contents in Rice Plants
Jian-Feng Jin, Qiang Gao, Yong Chen, Jun-Hui Wang*
College of Life Sciences, Zhejiang University, Hangzhou 310012, China
Abstract: In the present paper， using Agrobacterium-mediated transgene technique， an Arabidopsis anti-freeze transcriptional factor gene CBF1 was successfully introduced into japonic rice Zhonghua 11， and T1 generation of transgenic rice was achieved. The CBF1 gene and the selection gene HPT were detected in the T1 seedlings， and these genes were in Mendelian single-copy inheredity. Both under normal and low temperature， the proline contents in transgenic rice were increased; meanwhile， some low-temperature-tolerance phenotype was observed.
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