Effect of LncRNA XIST on High Glucose Induced Podocyte Injury through Targeted Regulation of miR-23a-3p

This article aims to explore the effect of lncRNA (long non coding RNA) XIST (X-inactive spe cific transcript) on HG (high glucose)-induced podocyte injury through targeted regulation of miR-23a-3p. Mouse renal podocyte line MPC5 was selected as the study subjects and randomly assigned into Control group (con ventional culture, 5 mmol/L glucose treatment), HG group (HG induction, 30 mmol/L glucose treatment), sh NC group (HG induction+transfection of sh-NC), sh-XIST group (HG induction+transfection of sh-XIST), sh XIST+anti-miR-NC group (HG induction+transfection of sh-XIST+anti-miR-NC), and sh-XIST+anti-miR-23a 3p group (HG induction+transfection of sh-XIST+anti-miR-23a-3p). qRT-PCR was used to detect the expression of LncRNA XIST and miR-23a-3p in cells. CCK8 and clone formation experiments were performed to detect cell proliferation. Transwell experiment was performed to detect cell migration. Flow cytometry was used to detect cell apoptosis rate. ELISA method was performed to detect IL-6, IL-10, GSH, and CAT in cells. Western blot was performed to detect the expression of PCNA, MMP-9, Bax, and Bcl-2 proteins in cells. Dual luciferase assay was used to detect the targeting relationship between LncRNA XIST and miR-23a-3p. The results showed that the HG group had higher LncRNA XIST level, apoptosis rate, the level of IL-6, and the expression level of Bax protein than the Control group (P<0.05), and lower miR-23a-3p level, cell survival rate, clone number, mi gration number, the levels of IL-10, GSH, CAT, the expression level of PCNA, MMP-9, and Bcl-2 proteins than the Control group (P<0.05). The sh-XIST group had lower LncRNA XIST level, apoptosis rate, the level of IL 6, and the expression level of Bax protein (P<0.05), and higher miR-23a-3p level, cell survival rate, clone num ber, migration number, the levels of IL-10, GSH, CAT, the expression level of PCNA, MMP-9, and Bcl-2 pro teins than the HG and sh-NC groups (P<0.05). The sh-XIST+anti-miR-23a-3p group had higher apoptosis rate, the level of IL-6, and the expression level of Bax protein (P<0.05), and lower miR-23a-3p level, cell survival rate, clone number, migration number, the levels of IL-10, GSH, CAT, the expression level of PCNA, MMP-9, and Bcl-2 proteins than the sh-XIST group and the sh-XIST+anti-miR-NC group (P<0.05). Compared with the XIST-WT+mimic-NC group, the XIST-WT+miR-23a-3p mimic group showed a conspicuous decrease in dual luciferase activity (P<0.05). The above results indicate that knocking down LncRNA XIST may promote miR-23a 3p, thereby inhibiting the progression of HG induced podocyte injury.

CSTR: 32200.14.cjcb.2025.10.0016