Observation on Spermatogenesis and Ultrastructure of Hirudo nipponia
QU Ruyu#, SHEN Hongying#, LI Qian, QIU Xia, LUO Yuanyuan*
Spermatogenesis and sperm morphology are important for animal reproduction, particularly in the cross-fertilizing invertebrates such as leeches. In this study, the spermatogenesis process and ultrastructural features of Hirudo nipponia were studied, this study will provide morphological support for investigating the reproductive biology of this species. The testis structure of Hirudo nipponia was observed by light microscopy, and the ultrastructure of spermatids in the specific region of testis was observed by TEM (transmission electron microscopy). The results showed that the spermatogenesis was consistent with the general mode of spermatozoa development, but showed some specificity in the distribution area of organelles and the degree of aggregation and coiling. This study clarified the structural changes of various organelles during the development of sperm in Hirudo nipponia, which provided new morphological knowledge for future study on germ cell development and fertilization mechanism of leeches, the findings will provide theoretical support for artificial breeding of leeches.
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