Effect of Sucrose on Cell Cycle Process

The growth of roots is achieved by cell proliferation and cell elongation, both of which are directly or indirectly regulated by cell cycle. Previous studies have shown that the lack of sucrose will affect the cell proliferation and cell elongation of Arabidopsis tap roots, and have a certain impact on the cell cycle. In this study, the growth differences of Arabidopsis seedlings under the conditions of 1% sucrose and no sucrose were analyzed, and the proliferation ability of root tips was indicated by EdU (5-ethynyl-2-deoxyuridine) staining, and the cell types were further detected by flow cytometry. The results showed that sucrose regulated the proliferation of Arabidopsis root tip cells, and sucrose was a signal to activate cell proliferation. When sucrose is deficient, the S phase of root tip cells is shortened, the proportion of G1 phase cells is obviously increased, and the ability of nuclear replication is decreased, which affects cell proliferation and cell elongation. This study proves that sucrose affects the G1-S phase transition and the ability of nuclear replication in the cell cycle of root tip, and then regulates cell proliferation and cell elongation, which provides further understanding for the regulation of root tip growth.

CSTR: 32200.14.cjcb.2025.05.0013