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Germinal Vesicle Transfer of Mammalian Oocytes
Long-Bo Cui*, Fang-Zhen Sun1
Department of Biochemistry, Yantai University, Yantai 264005, China;1Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100080, China
Abstract: Germinal vesicle (GV) transfer is a technique that GV in GV oocyte is removed and inserted into the perivitelline space of the enucleated oocyte (GV oocyte， MII oocyte or fertilized egg)， forming a reconstructed oocyte by fusion. GV transfer may be a valuable research procedure that generates cell models to characterize the cytoplasmic-nuclear interplay for cell cycle regulation， maturation， and fertilization in oocytes. It can be used to study the relationship between meiotic errors and aged-related changes in the oocytes and between oocytoplasmic ageing and oocyte aneuploidy. This paper reviews general procedure of GV transfer， fusion of GV-karyoplast and cytoplast， cultural conditions of the reconstructed oocytes， fertilization， artificial activation and developmental capacity of the matured reconstructed oocytes and significance of GV transfer.
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