Exploration and Practice of Interdisciplinary Mixed Teaching Mode of Macrophage Phagocytosis Experiment

Phagocytosis experiment is a classic experiment of cell biology, immunology and microbiology. However, the current experimental teaching rarely considers the integration of these disciplines in content design, and there are few applications of virtual experiments in the teaching of phagocytic experiments. The teaching team of the author introduced mouse macrophage line RAW264.7 as the experimental object and fluorescent labeled Escherichia coli BL21 (pET-28a-mCherry) as the tracer to explore the changes of cell morphology and phagocytic function before and after classical activation of macrophages. According to the characteristics of the knowledge field of the students, the content of cell biology is designed as the main line, and the disciplines of immunology and microbiology are used as auxiliary and supplement. With the help of online learning platform and the college’s high-quality virtual experiment platform, online learning content closely related to offline teaching is provided for students; at the same time, this team adopted the diversified mixed teaching mode combined traditional classical classroom teaching with semi-open experimental teaching, and established a multi-dimensional evaluation system. Through the implementation of these teaching reform measures, the knowledge points of cell biology, immunology, microbiology and other disciplines related to phagocytosis are organically integrated, aiming to enable students to comprehensively understand phagocytosis, which is a life activity, effectively stimulate students’ awareness of innovation and exploration, and expand the experimental teaching mode.

CSTR: 32200.14.cjcb.2025.04.0012