Impact of Syringin on the Fibrosis of Cirrhotic Rats by Regulating Hedgehog-YAP1 Signal Pathway
WU Jiande1, YANG Qiu2, XIE Guidan1, PENG Xin1*
In order to investigate the impact of syringin on the fibrosis of cirrhotic rats by regulating Hedgehog-YAP1 (Yes-associated protein 1) signal pathway, SD rats were randomly divided into model group, low-dose syringin group, high-dose syringin group, Purmorphamine (Hedgehog activator) group, high-dose syringin+Purmorphamine group, another rats were selected as the control group, the liver index and liver function indexes [ALT (alanine aminotransferase) and AST (aspartate aminotransferase)] were measured after treatment with syringin and Purmorphamine; the hepatic fibrosis was detected by Masson staining in cirrhotic rats; the content of HYP (hydroxyproline) in liver tissue, and the levels of TNF-α, IL-6, MDA and TAC (total antioxidant capacity) in liver tissue and serum of cirrhotic rats in each group were measured with the kit; Western blot was used to detect the expression of collagen I, collagen Ⅲ and Hedgehog-YAP1 signal related protein in liver tissues of cirrhotic rats in each group. The results show that, compared with the control group, the liver index, the levels of ALT and AST, the levels of TNF-α, IL-6, and MDA in serum, the content of HYP in liver tissue, the proportion of collagen fibers in liver tissue, the levels of TNF-α, IL-6, and MDA in liver tissue, the expression of collagen I, collagen Ⅲ, Hedgehog proteins in liver tissue and p-YAP1/YAP1 in liver tissue in model group increased (P<0.05), while the level of TAC in serum and in liver tissue decreased (P<0.05). Compared with the model group, the levels of TNF-α, IL-6, and MDA in serum, the content of HYP in liver tissue, the proportion of collagen fibers in liver tissue, the levels of TNF-α, IL-6, and MDA in liver tissue, the expression of collagen I, collagen Ⅲ, Hedgehog proteins in liver tissue and p-YAP1/YAP1 in liver tissue in the low-dose and high-dose syringin groups decreased (P<0.05), the level of TAC increased (P<0.05), and the effect of high dose syringin is stronger. The change trend of each index of rats in the Purmorphamine group is contrary to that in the low and high dose groups of syringin, and Purmorphamine can reverse the effect of syringin on each index of rats in the model group. This indicates that syringin can inhibit inflammation and oxidative stress by blocking the activation of Hedgehog-YAP1 signal, thereby reducing liver fibrosis and improving liver function in cirrhotic rats.
CN
EN