Effect of Physalis Calyx Seu Fructus Alcoholic Extract on Ferroptosis of Breast Cancer Cells by Regulating SLC7A11/ALOX12 Pathway
SUN Chengyang1, YUAN Shibo1, WANG Yunliang2*, LÜ Tianxu3, BAO Dalin4, SUN Hongmei5
This study aimed to explore the effect of PCF (physalis calyx seu fructus) alcohol extract on ferroptosis of breast cancer cells through the SLC7A11 (solute carrier family 7 member 11)/ALOX12 pathway. This study selected breast cancer MCF-7 cell lines and assigned into five groups: control group, low concentration PCF group, medium concentration PCF group, high concentration PCF group, high concentration PCF+activator group. EdU detection, lipid peroxidation detection, iron determination, and GSH (glutathione) content determination experiments were conducted; qRT-PCR was used to detect the mRNA expression of SLC7A11 and ALOX12 in cells. In addition, Western blot was used to detect the expression of SLC7A11 and ALOX12 proteins in cells. The results showed that compared with the control group, the EdU positive rate, GSH content, SLC7A11 mRNA and protein expression in MCF-7 cells were reduced in the low, medium, and high concentration PCF groups, the degree of lipid peroxidation, ferrous and total iron ion contents, ALOX12 mRNA and protein expression were elevated (P<0.05), and the effect of PCF alcohol extract on MCF-7 cells increased with increasing concentration (P<0.05). Compared with the high concentration PCF group, the EdU positive rate, GSH content, SLC7A11 mRNA and protein expression in MCF-7 cells were raised in the high concentration PCF+activator group, the degree of lipid peroxidation, ferrous and total iron ion contents, ALOX12 mRNA and protein expression were reduced (P<0.05). The addition of ferroptosis inhibitor Ferrostatin-1 reversed the effect of PCF ethanol extract on ferroptosis of breast cancer cells (P<0.05). PCF ethanol extract may promote ferroptosis of breast cancer cells by regulating SLC7A11/ALOX12 pathway.
CN
EN