The Impacts of Astragaloside IV on the Proliferation and Migration of Human Keloid Fibroblasts by Regulating the Notch Signaling Pathway
JIANG Zhiyong, XI Qingchun, LI Xuan, SANG Pengfei*
This study aims to investigate the impacts of astragaloside IV on the proliferation and migration of HKF (human keloid fibroblast), and to analyze whether its mechanism is related to the Notch signaling pathway. HKF was assigned into NC group (normal culture), L-astragaloside IV group (25 μmol/L astragaloside IV), M-astragaloside IV group (50 μmol/L astragaloside IV), H-astragaloside IV group (100 μmol/L astragaloside IV), and H-astragaloside IV+Notch signaling pathway activator Jagged1-Fc group (100 μmol/L astragaloside IV+0.5 μg/mL Jagged1-Fc group). CCK-8 method, flow cytometry, scratch assay, Transwell method, and Western blot separately were used to detect the proliferation, apoptosis, migration, invasion, and the expression of PCNA, MMP-2, collagen-I, collagen-III, Notch1, and Hes1 proteins in HKF. The D value of cells, scratch healing rate, number of invasive cells, expression of PCNA, MMP-2, collagen-I, collagen-III, Notch1, and Hes1 proteins in L-astragaloside IV group, M-astragaloside IV group, and H-astragaloside IV group were lower than those in NC group, and the apoptosis rate was higher (P<0.05). The D value, scratch healing rate, number of invasive cells, expression of PCNA, MMP-2, collagen-I, collagen-III, Notch1, and Hes1 proteins in H-astragaloside IV+Jagged1-Fc group were prominently higher than those in H-astragaloside IV group, and the cell apoptosis rate was prominently lower (P<0.05). Astragaloside IV may inhibit the proliferation and migration of HKF by suppressing the Notch signaling pathway.
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