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Isolation and Culture of Human Pleural Mesothelial Cells
Hong-Feng Wang, Wen-Xiu Qu, Hong-Bo Liu, Ping He, Sheng-Qi Li*
Department of Respiratory Medicine, Second Hospital (Shengjing Hospital), China Medical University, Shenyang 110004, China
Abstract: Use two methods to establish human pleural mesothelial cells (HPMCs) culture reproducible model in vitro and compare the advantage and disadvantage of them. Mesothelial cells were isolated from human pleura by trypsin EDTA disaggregation and pleural effusion fluid. HPMCs were identified by morphology and immunohistochemstry: Streptaridin peroxidase conjugated method. Confluent HPMCs appeared multipolar and like cobblestone; Numerous surface microvilli and abundant endoplasmic reticulum were observed under electron microscopy. HPMCs expressed cytokeratin and vimentin; VIII factor associated antigen and CD45 were negative. Two methods achieved success in establishment of reproducible model for culture of HPMCs. Those methods were feasibility in methodology. The cells isolated from human pleura had higher purity quotient. Pleural effusion fluid was easier to obtain.
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