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Induction of ROS/RNS Dynamics in the Macrophage by Ox-LDL
Tong-Le Deng, Le Zhang, Ya-Kun Ge, Ke-Di Xu, Xiao-Xiang Zheng*
Department of Biomedical Engineering, Zhejiang University, Hangzhou 310027, China
Abstract: Effect of Ox-LDL on kinetic changes of ROS/RNS in U937-derived macrophages was investigated. In the present study， detecting dynamics of intracellular NO， O2·- and ROS under the acute and chronic exposure of U937-derived macrophages to Ox-LDL， we found that Ox-LDL significantly induced the increase of intracellular NO and O2·- in time-dependent manner， and that increase of intracellular NO was significantly higher than of O2·- in the acute exposure. Moreover， in chronic exposure， Ox-LDL significant induced the increase of ROS at 12 h， but at 24 h ROS levels significantly decreased than at 12 h (P<0.01) and significantly higher than control cells (P<0.01). The findings suggest that modulation of free radicals metabolism may be a potential therapeutic strategy against atherosclerotic inflammation in different phases of early foam cells.
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