Progress in Research Methods of Human Blood Proteomics

YANG Yuping¹, HAN Shengcheng¹, HUANG Rui^2,3*

(¹ Beijing Key Laboratory of Gene Resources and Molecular Development, Beijing Normal University, Beijing 100875, China; ² Key Lab of Stomatology of State Ethnic Affairs Commission, Northwest Minzu University, Lanzhou 730030, China; ³ Key Laboratory of Environmental Ecology and Population Health in Northwest Minority Areas, Northwest Minzu University, Lanzhou 730030, China)

Abstract:

Blood proteomics plays a crucial role in biomedical research in the post-genomic era and has attracted much attention as an important field for studying biomarkers. Through high-throughput proteomics detection techniques, various protein substances in plasma/serum can be analyzed, including the total proteome and posttranslational modifications of proteins, providing assistance for the discovery of new biomarkers and the development of clinical diagnostic methods. This article reviews the background and progress of blood proteomics research, focuses on introducing two major types of protein identification and analysis methods based on mass spectrometry, namely LC-MS/MS technology and affinity-based Olink and SomaScan technologies, discusses the latest research and application progress in the field of blood proteomics, and looks forward to its future development trends.

CSTR: 32200.14.cjcb.2025.02.0012