The Study on Intervention of Acetylated Modification Mediated by Different Forms of Aerobic Exercise on Renal Tumor in Mice

MAO Haifeng^1,2, CHEN Jiaqin^3*, ZHOU Wenyan², ZHU Jingsheng³, CHEN Weiwei³, LI Wenmin³, ZHOU Bocun³

(¹Department of Physical Education of Suqian University, Suqian 223800, China; ²Department of Physical Education of Yichun University, Yichun 336000, China; ³Department of Physical Education of Hunan Normal University, Changsha 410012, China)

Abstract:

This study aimed to explore the intervention effect and possible mechanism by different forms of aerobic exercise mediating acetylation modification on mouse kidney tumors. Forty healthy male KM mice were used to construct the model of cancer cell invasion into renal capsule. Then, they were randomly divided into four groups: model control group (M), treadmill group (TM), swimming group (SW), heavy load swimming group (HL), and another 10 healthy male KM mice were taken as blank group (B) without cancer cell invasion. According to the exercise program, three exercise groups took aerobic exercise. After the end of the exercise, tumor tissue were taken out, weighted, and the tumor inhibition rate was calculated. Tumor and renal tissue’s microscopic morphology was observed under the light microscope by HE staining; expression of P300 and Tip60 proteins were detected by immunohistochemical staining; HDAC2, HDAC3 and HDAC8 mRNA expression was detected by qRT-PCR. The results showed that the tumor inhibition rate of group SW was the highest, followed by the group TM and the group HL; the degree of renal interstitial injury of group M was the highest, and the renal interstitial injury of three exercise groups were decreased in different degree; the tumor cells damage of group M was the lowest, and the tumor cells damage of three exercise groups were increased in different degrees; the P300 protein expression of group HL was the highest, followed by group M, group SW, group TM, group B; the Tip60 protein expression of group HL was the lowest, followed by group M, group SW, group TM, group B; the HDAC2 mRNA expression of group B was the lowest, followed by group TM, group SW, group M, group HL; the HDAC3 mRNA expression was similar to HDAC2, but the expression of group HL was lower than that of group M; the HDAC8 mRNA expression was the same as HDAC3. In conclusion, medium and small intensity treadmill or no load swimming exercise has a certain inhibitory effect on the tumor. The acetylation level of histones can be regulated by histone acetyltransferase and histone deacetylase, thereby affecting the generation and inhibition of tumor cells.

CSTR: 32200.14.cjcb.2025.02.0003