Discovery and Analysis of Folate Anabolism Genes during Grain Development in Minshuangse No.6
ZHAN Penglin, ZHANG Yang, LIN Jianxin, XU Jin, ZHUANG Wei, LU Heding, CHEN Shanhu, LIAO Changjian*
Folic acid is an essential vitamin for human body. The sweet maize is one of most important cereal crop, which has fruit, vegetable and food functional characteristic. However, the folate content and regulation genes are still unclear in sweet maize. The different kernel development stages of Minshuangse No.6 were used as materials to determine folate content and genes expression. The different expression genes were performed by Mfuzz time Cluster analysis. The Cluster genes were studied by GO and KEGG enrichment analyses, aiming toexplore potential folate synthesis genes and molecular regulatory pathways. The results showed that folate content in sweet maize decreased gradually with the increase kernel development time. The folic acid content were above 50 μg/100 g from 10 to 20 days after pollination, and showed a rapid decline after 20 days. This study obtained six Clusters by Mfuzz time Cluster analysis, among which Cluster 3 gene expression pattern had a similar trend with folate content change. The GO and KEGG enrichment analyses also revealed that the folate biosynthesis and metabolism pathways were enriched by the Cluster 3. This study found 19 genes had involved in folate biosynthesis in sweet maize, among which seven genes were involved in GTP (guanosine triphosphate) as a substrate for processing new compounds. In addition, the expression pattern of Zm00001d031995 (DHNA), Zm00001d018733, Zm00001d023817 (DHFS) and Zm00001d026549 was consistent with the change trend of folate content. Meanwhile, the expression of Zm00001d039264 and Zm00001d016866 had opposited trend with folate content. Therefore, these six genes maybe play a key role in the regulation of folate molecular pathways, which provides genetic resources for high-folate breeding in sweet maize.
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