Effects of circ_0000467 on Proliferation, Migration and Invasion of Ovarian Cancer Cells by Targeting miR-508-3p
QI Lining1, WANG Qi1, ZHU Jihong1, GENG Yanhong1, GE Xinmiao2, WEI Xujing3*, LIU Caihui1
This study aims to explore the molecular mechanism of circ_0000467 regulating the proliferation, migration and invasion of ovarian cancer SKOV3 cells. The ovarian cancer tissues and their adjacent tissues of 37 patients from the Baoding No.1 Central Hospital from March 2021 to June 2022 were collected. IOSE80 human normal ovarian epithelial cell lines and human ovarian cancer cells A2780, OVCAR3 and SKOV3 were cultured in vitro. The expression levels of circ_0000467 and miR-508-3p were detected by qRT-PCR, and the correlation between the expression levels of circ_0000467 and miR-508-3p and clinicopathological characteristics of ovarian cancer patients was analyzed. si-circ_0000467, si-NC, miR-NC, miR-508-3p were transfected into SKOV3 cells, and si-circ_0000467 was co-transfected with anti-miR-NC and anti-miR-508-3p into SKOV3 cells, respectively. Proliferation was measured by CCK-8 and plate clone formation assay. The cell migration was measured by scratch test. The cell invasion was measured by Transwell assay. The effects of miR-508-3p overexpression on the luciferase activity of wild-type vector WT-circ_0000467 and mutant vector MUT-circ_0000467 were detected by dual luciferase assay. Compared with adjacent tissues, circ_0000467 was up-regulated in ovarian cancer tissues (P<0.05) and miR-508-3p was down-regulated (P<0.05). Compared with IOSE80 cells, the expression level of circ_0000467 in A2780, OVCAR3 and SKOV3 cells was increased (P<0.05), while the expression level of miR-508-3p was decreased (P<0.05). circ_0000467 and miR-508-3p expression were correlated with FIGO stage and lymph node metastasis in ovarian cancer patients (P<0.05). Knocking down circ_0000467 could reduce the cell viability, scratch healing rate, the number of cell clones and the number of invasive cells (P<0.05). Overexpression of miR-508-3p could reduce the luciferase activity of WT-circ_0000467 (P<0.05). Transfection with miR-508-3p decreased the cell viability, scratch healing rate, the number of cell clone formation and the number of invasive cells (P<0.05). Transfection with anti-miR-508-3p reversed the effects of transfection with si-circ_0000467 on cell proliferation, migration and invasion (P<0.05). Interference with the expression of circ_0000467 could inhibit the proliferation, migration and invasion of ovarian cancer cells by increasing the expression of miR-508-3p.
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