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Increased Migration of 6T-CEM through Human Brain Microvascular Endothelial Cells Monolayer Dependent on Macrophage Inflammatory Protein-1a
De-Shu Shang, Yi-Ran Ma, Wei-Dong Zhao, Wen-Gang Fang, Li Zhu, Yu-Hua Chen*, Jin-Dan Song
Department of Developmental Biology, Key Laboratory of Cell Biology, Ministry of Public Health, the College of Basic Medical Science, China Medical University, Shenyang 110001, China
Abstract: It has been showed that T lymphocytes might participate in the inflammation process in Alzheimer's disease (AD)， however， it is unclear how circulating T cells cross the blood-brain barrier (BBB). We have showed the stronger ability to migrate through human brain microvascular endothelial cells (HBMECs) and a significantly higher macrophage inflammatory protein-1a (MIP-1a) expression in peripheral T lymphocytes of AD patients than age-matched healthy subjects. In order to explore the mechanism of the transendothelial migration of T lymphocytes further， rhMIP-1a or 6T-CEM， the cell line of human lymphoblastic leukemia which highly expressed MIP-1a， were incubated with HBMEC monolayer separately. It was showed that rhMIP-1a could enhance the migration of 6T-CEM cells through HBMEC monolayer and disrupt the tight junction of HBMEC. The expressions of CC chemokine receptor 5 (CCR5) on HBMECs which incubated with rhMIP-1a or 6T-CEM cells were detected. These data suggested that MIP-1a might promote the transendothelial migration of T lymphocytes by interacting with CCR5 on HBMEC monolayer.
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