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Suspension Culture Technology of Mammalian Cells and Its Application

CHEN Mingjie¹, BI Haiyang¹, MA Yizhao¹, ZHANG Shenghua¹, ZHANG Yanyan¹, ZHANG Yanru¹, YANG Songbai¹, ZHOU Xiaolong¹, QIAO Zilin², LI Xiangchen¹, WU Yongshu¹*

(¹Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, Zhejiang Provincial Engineering Laboratory for Animal Health Inspection & Internet Technology, Zhejiang International Science and Technology Cooperation Base for Veterinary Medicine and Health Management, China-Australia Joint Laboratory for Animal Health Big Data Analytics, College of Animal Science and Technology & College of Veterinary Medicine of Zhejiang A&F University, Hangzhou 311300, China;²Gansu Technology Innovation Center of Animal Cell, Northwest Minzu University, Lanzhou 730030, China)

Abstract:

Suspension culture technology involves suspending cells in a culture medium, which is uti lized for virus propagation or protein expression. This technology is often utilized for purposes such as virus proliferation or protein expression. It has several advantages, including scalability for scale-large cell culture, high expression levels of target products, high quality of products and advantage for industrial application. Vari ous cell types have been successfully adapted to suspension cultures through the use of suitable bioreactors, mi crocarriers, and serum-free media. These cells are currently being utilized in a range of applications, including inactivated virus vaccine production, recombinant proteins and cell therapy. This review provides an in-depth analysis of mammalian cell suspension culture domestication technology and its applications in the fields of in activated virus vaccines, recombinant proteins, and cell therapy. By providing insights into the development and application of suspension culture technology, this review aims to serve as a valuable resource for researchers in this field.

CSTR: 32200.14.cjcb.2024.09.0011