Effect of Oleuropein on Alveolar Epithelial Cell Injury Induced by Streptococcus pneumoniae Infection by Regulating the RhoA/ROCK Signaling Pathway
YAN Yafen, LU Guangbing, TIAN Liangdong, ZHANG Dongxiang*
The aim of this study is to investigate the effect of OP (oleuropein) on alveolar epithelial cell injury induced by Streptococcus pneumoniae infection by regulating RhoA (Ras homologous gene family member A)/ROCK (Rho-associated coiled-coil kinase) signaling pathway. Alveolar epithelial cells A549 were randomly divided into Con trol group, Model group, L-OP group, M-OP group, H-OP group and U46619 group (H-OP+RhoA/ROCK signaling pathway activator U46619). Except for the normal culture of the Control group, the cell injury model was estab lished with Streptococcus pneumoniae infection in the other groups. MTT and plate cloning were used to detect the proliferation of cells. The levels of SOD (superoxide dismutase), ROS (reactive oxygen species) and MDA (malo ndialdehyde) were detected by kits. The levels of IL-10 (interleukin-10), IL-1β and TNF-α (tumor necrosis factor-α) were detected by ELISA. Flow cytometry was used to detect apoptosis. Western blot was used to detect the expression of Bcl-2, Bax, cleaved caspase-3, RhoA and ROCK. The result showed that compared with the Control group, the D490, clone number, the expression levels of SOD, IL-10 and Bcl-2 in the Model group were decreased; the apoptosis rate, the expression levels of ROS, MDA, IL-1β, TNF-α, Bax, cleaved caspase-3, RhoA and ROCK were increased. Com pared with the Model group, the D490, clone number, the expression levels of SOD, IL-10 and Bcl-2 in L-OP group, M-OP group and H-OP group were increased; the apoptosis rate, the expression levels of ROS, MDA, IL-1β, TNF-α, Bax, cleaved caspase-3, RhoA and ROCK were decreased. Compared with the H-OP group, the D490, clone number, the expression levels of SOD, IL-10 and Bcl-2 in the U46619 group were decreased; the apoptosis rate, the expression levels of ROS, MDA, IL-1β, TNF-α, Bax, cleaved caspase-3, RhoA and ROCK were increased. As a reminder, OP may alleviate inflammation and oxidative stress in alveolar epithelial cells infected with Streptococcus pneumoniae by inhibiting the RhoA/ROCK signaling pathway, thereby promoting cell proliferation, inhibiting cell apoptosis, and al leviating cell injury.
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