Expression of LINC01128 in Endometrial Cancer Tissue and Its Effect on Cell Malignant Progression by Regulating miR-367-3p/KLF5 Axis
FU Qianqian1, SHAO Fengxia2*, HUANG Xiaoyan1
This study aims to investigate the expression of LINC01128 in EC (endometrial cancer) tissue and its effect on cell malignant progression by regulating the miR-367-3p/KLF5 (Krüppel like factor 5) axis. qRT-PCR was ap plied to detect the expression levels of LINC01128, miR-367-3p, and KLF5 mRNA in EC tissue, cell line HEC-1A, human endometrial epithelial cells CP-H058. HEC-1A cells cultured in vitro were randomly separated into five groups: NC group (normal culture), si-LINC01128 group, si-NC group, miR-367-3p mimic group, and miR-NC group. And the si-NC group, si-LINC01128 group, miR-367-3p mimic group, and miR-NC group were transfected with si-NC, si-LINC01128, miR 367-3p mimic, and miR-NC, respectively. CCK-8 method and TUNEL staining were applied to detect the proliferation and apoptosis of HEC-1A cells, respectively. Transwell and scratch experiments were applied to detect the invasion and migra tion of HEC-1A cells, respectively. Dual luciferase reporter gene experiment was applied to verify the targeting relationship between LINC01128 and miR-367-3p, and between miR-367-3p and KLF5. Western blot was applied to detect the ex pression of KLF5 in cells. Compared with adjacent tissues, the expression of LINC01128 and KLF5 mRNA in EC tissues was increased, while miR-367-3p expression was decreased; compared with CP-H058 cell, the expression of LINC01128 and KLF5 mRNA in EC cell HEC-1A was increased, while miR-367-3p expression was reduced (P<0.05). There was no significant difference in the expression of LINC01128, miR-367-3p, KLF5 mRNA, as well as HEC-1A cell proliferation, apoptosis, migration, and invasion between the NC group and the si-NC group (P>0.05). Compared with the si-NC group, the expression of LINC01128 and KLF5 mRNA, D450 value, migration rate, invasion number, and Bcl-2 expression in the si-LINC01128 group were all reduced, while miR-367-3p expression, apoptosis rate, and Bax expression were all increased (P<0.05). There was no great difference in the expression of LINC01128, miR-367-3p, KLF5 mRNA, as well as HEC-1A cell proliferation, apoptosis, migration, and invasion between the NC group and the miR-NC group (P>0.05). Compared with the miR-NC group, there was no great difference in LINC01128 expression in the miR-367-3p mimic group (P>0.05), the KLF5 mRNA expression, D450 value, migration rate, invasion number, and Bcl-2 expression were all reduced, while miR-367-3p expression, apoptosis rate, and Bax expression were all increased (P<0.05). The results of dual luciferase reporter gene experiment showed that miR-367-3p had targeted regulatory relationship with LINC01128 and KLF5. LINC01128 is highly expressed in EC tissues and cells, and interference with LINC01128 can inhibit malignant progres sion of EC by regulating the miR-367-3p/KLF5 axis.
CN
EN