Analysis of Cross-Contamination in 54 Human Lung Cancer Cell Lines
FANG Weiying*, LIU Yi, XU Lan, GU Tingyu, CHEN Yuelei
This study aimed to authenticate the correctness of human lung cancer cell lines commonly used in Chinese labs. Genomic DNA was extracted from 54 human lung cancer cell samples collected from several domestic labs, STR (short tandem repeat) sequences were amplified by PCR, and capillary electrophoresis was performed to obtain the STR profiles of these cells. When compared to their known STR profiles released in the international databases, the identity information of these cell lines was confirmed by the matching rate, and cell cross-contamination was determined. The results showed that 14 of the 54 cell lines were cross-contaminated, with an error rate of 25.93% (14/54). The error rate of 51 common human lung cancer cell samples was 27.45% (14/51), and the error rate of three samples of human lung cancer cell lines established by the scientists in Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences was 0% (0/3). The causes of cross-contamination of cell lines in Chinese labs were analyzed, and solutions for this serious phenomenon were proposed.
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